Running medium chromatography

Thin layer chromatography - Running medium HPLC

(Thin layer) chromatography is a method of separating a mixture using the mobile and stationary phases. The mobile phase is one or more solvents, the so-called running medium. In chromatography, the layer on a support plate is called the stationary phase. It usually consists of aluminum oxide or silica gel. The sample to be analyzed is applied as a solution to the layer of a carrier plate and this is introduced vertically together with the running medium into the chamber of the HPLC column. The running medium moves upwards on the plate at a certain speed and ensures the desired separation effect for the substance in question. Subsequently, the individual particles or molecules can be identified by means of the retention factor (Rf value). In order to achieve an optimum result for each substance, a wide range of different running agents with various degrees of purity is available for the mobile phase. This makes it possible to adequately analyze any stationary phase or sample

The right solvent for the HPLC column

The solvent of the respective substance is generally the most suitable solvent for use as a mobile phase. If this is not possible, the flow agent and solvent should at least have a similar structure or be easily miscible. Which superplasticizer should optimally be used in thin-layer chromatography also depends on several factors, such as the HPLC column used. The detector used also plays a role. For example, UV detectors require superplasticizers that do not show residual absorption at the wavelength used so as not to negatively affect the detection of the analyzed substances. For example, acetonitrile mixed with water, as opposed to methanol, is suitable for use in areas where a lower viscosity is required. Acetonitrile also has a comparatively high eluting power. Methanol, on the other hand, has a lower toxicity and is less expensive to purchase. While acetonitrile is suitable for use with UV detectors that measure in ranges of 200 nm or less, the range for methanol is above 230 nm

Running Agents for Laboratory Analytics - buy online at WICOM

At WICOM you will find an extensive range of running agents for every need in the field of HPLC. WICOM offers you not only suitable superplasticizers, but also many other products for chromatography in our online store.